Object Oriented Evolution

Apr 20 2010

Inferring Heterozygosity from SNP data

This looks straightforward. 

n = total number of reads aligning to a base

y = total number of reads aligning to a base that match the reference

p = probability that reads match the reference base

Bases with LOD>3 were called SNPs or sites of heterozygosity.

From Bloom et al. (2009) Measuring differential gene expression by short read sequencing: quantitative comparison to 2-channel gene expression microarrays. BMC Genomics vol. 10 (1) pp. 221

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